Vol 2-6 Commentary

Immune Thrombocytopenia: Antiplatelet Autoantibodies Inhibit Proplatelet Formation by Megakaryocytes and Impair Platelet Production in vitro

José Perdomo

Haematology Research Unit, St George and Sutherland Clinical School, Faculty of Medicine, University of New South Wales, Sydney, NSW, Australia

DOI: 10.29245/2578-3009/2018/6.1163 View / Download Pdf View Full Text
Vol 2-6 Commentary

Commentary: "Interference of Doxycycline Pretreatment in a Model of Abdominal Aortic Aneurysms"

Karina M. Mata1*, Cleverson R. Fernandes1, Cristiane Tefé-Silva2, Simone G. Ramos1

1Department of Pathology, Ribeirão Preto Medical School, University of São Paulo, São Paulo, Brazil

2University Center of Barão de Mauá, Ribeirão Preto, SP, Brazil

Abdominal aortic aneurysm (AAA) represents a complex pathophysiological process of weakening and dilatation of the aortic wall associated with atherosclerosis, chronic inflammatory response and hemodynamic alterations. Degradation of the extracellular matrix by the matrix metalloproteinases (MMPs) and an imbalance between MMPs and their tissue inhibitors (TIMPs), have fundamental roles in the development of AAA. However, the exact pathogenetic mechanisms remain incompletely elucidated. In addition to the previous results already published “Interference of doxycycline pretreatment in a model of abdominal aortic aneurysms”, in this commentary we have complementary results. Here we have included new findings of the TIMPs 1 and 2 expressions in animals submitted to AAA surgical induction associated with doxycycline pretreatment. In this study, we used a new experimental model, developed in our laboratory, to induce AAA by combining two potential causes of MMP secretion: inflammation and turbulent blood flow. Male Wistar rats were divided into Control (C), Control+ Doxycycline (C+D), Aneurysms (A) and Aneurysms+Doxycycline (A+D) groups. The rats were euthanized at 3, 7 or 15 days post-surgery (dps). The administrated doxycycline started 48 hours before the surgical induction of AAA until the end of the experiment. After 1, 3, 7, and 15 dps, the animals were euthanized under anesthesia and the vessels were collected to measurement of TIMPs 1 and 2 by western blot. Our results demonstrate an increased expression of TIMPs 1 and 2 in aneurysm group (A) probably in an attempt to counteract the increased activity of MMPs 2 and 9. In aneurysms groups submitted to doxycycline pretreatment (A+D) showed the regulation of expression of TIMP 1 and 2, remaining close to baseline levels from the third day, similar to expression found the control groups (C and C + D). This study suggests that the pretreatment with doxycycline balances the TIMPs 1 and 2 expressions with a protective effect on the progression of abdominal aortic aneurysms in experimental model.

DOI: 10.29245/2578-3009/2018/6.1155 View / Download Pdf View Full Text